期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 25, 期 1, 页码 809-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41594-017-0006-4
关键词
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资金
- Royal Society Darwin Trust Research Professorship
- Wellcome Trust Senior Investigator Award [103792]
- Wellcome Trust Programme Grant [092545]
- Herchel Smith Research Studentship
- Wellcome Trust [092096]
- CRUK [C6946/A14492]
Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are still unknown. One of the mechanisms by which lncRNAs function is through the modulation of chromatin states. To assess the functions of lncRNAs, we developed RNA-DamID, a novel approach that detects lncRNA-genome interactions in a cell-type-specific manner in vivo with high sensitivity and accuracy. Identifying the cell-type-specific genome occupancy of lncRNAs is vital to understanding their mechanisms of action in development and disease. We used RNA-DamID to investigate targeting of the lncRNAs in the Drosophila dosage-compensation complex (DCC) and show that initial targeting is cell-type specific.
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