期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 12, 期 13, 页码 1532-1537出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201700340
关键词
aggregation-caused quenching; fluorescent probes; FRET; naphthalimides; two-photon probes
资金
- National Natural Science Foundation of China [21472172, 21272212]
- Natural Science Foundation of Zhejiang Province [LY17B060009]
- Project of Science Technology Department of Zhejiang Province [2014C33141]
- Project of Department of Science Technology Jinhua City [2013-3-003]
- National University of Singapore [R279-000-482-133]
A single fluorescent signal output often does not satisfy the detection accuracy requirements for bioactive compounds in biological systems. It is of great interest to develop a dual-channel turn-on fluorescence sensing system for self-validated detection. Herein, we report a self-quenching nanoparticle composed of dual two-photon dyes of naphthalimides, displaying almost no fluorescence at both channels, owing to aggregation-caused quenching (ACQ). A dual two-photon-channel fluorescent probe for GSH was further developed by inserting a disulfide bond between two naphthalimides. Upon treatment with GSH, the disulfide bond was cleaved, and two napthalimide fluorophores were separated, leading to turn-on fluorescence at both channels. This dual-two-photon-channel design strategy can be easily extended to other analytes, simply by changing the linker molecules, which creates new opportunities to accurately monitor various biological processes in living cells.
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