期刊
ANALYTICAL CHEMISTRY
卷 89, 期 13, 页码 7038-7045出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00754
关键词
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资金
- National Natural Science Foundation of China [U1608222]
- State Key Laboratory of Fine Chemicals [KF1606]
A novel lysosome-targeting ratiometric fluorescent probe (CQ-Lyso) based on the chromenoquinoline chromorphore has been developed for the selective and sensitive detection of intracellular pH in living cells. In acidic media, the protonation of the quinoline ring of CQ-Lyso induces an enhanced intramolecular charge transfer (ICT) process, which results in large red-shifts in both the absorption (104 nm) and emission (53 nm) spectra which forms the basis of a new ratiometric fluorescence pH sensor. This probe efficiently stains lysosomes with high Pearson's colocalization coefficients using LysoTrackerDeep Red (0.97) and LysoTrackerBlue DND-22 (0.95) as references. Importantly, we show that CQLyso quantitatively measures and images lysosomal pH values in a ratiometric manlier using single-wavelength excitation.
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