期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 45, 期 6, 页码 1842-1854出版社
WILEY
DOI: 10.1002/eji.201445211
关键词
AHR; Helminth; IL-10; Regulatory Bcells; TLR7 Treg cells
类别
资金
- Science Foundation Ireland
- National Children's Research Centre
Bcells have been described as having the capacity to regulate cellular immune responses and suppress inflammatory processes. One such regulatory B-cell population is defined as IL-10-producing CD19(+)CD1d(hi) cells. Previous work has identified an expansion of these cells in mice infected with the helminth, Schistosoma mansoni. Here, microarray analysis of CD19(+)CD1d(hi) Bcells from mice infected with S. mansoni demonstrated significantly increased Tlr7 expression, while CD19(+)CD1d(hi) Bcells from uninfected mice also demonstrated elevated Tlr7 expression. Using IL-10 reporter, Il10(-/-) and Tlr7(-/-) mice, we formally demonstrate that TLR7 ligation of CD19(+)CD1d(hi) Bcells increases their capacity to produce IL-10. In a mouse model of allergic lung inflammation, the adoptive transfer of TLR7-elicited CD19(+)CD1d(hi) Bcells reduced airway inflammation and associated airway hyperresponsiveness. Using DEREG mice to deplete FoxP3(+) T regulatory cells in allergen-sensitized mice, we show that that TLR7-elicited CD19(+)CD1d(hi) Bcells suppress airway hyperresponsiveness via a T regulatory cell dependent mechanism. These studies identify that TLR7 stimulation leads to the expansion of IL-10-producing CD19(+)CD1d(hi) Bcells, which can suppress allergic lung inflammation via T regulatory cells.
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