期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 45, 期 4, 页码 1270-1273出版社
WILEY
DOI: 10.1002/eji.201445247
关键词
CD4(+) T cells; Cellular activation; Cytokines; Flow cytometry; PMA; ionomycin
类别
资金
- German Research Council [DFG SFB633, SFB 650, GRK1121]
- European Research Council [268987]
- European Research Council (ERC) [268987] Funding Source: European Research Council (ERC)
Restimulation of memory-effector T helper lymphocytes with PMA/ionomycin is an established technique to determine their imprinting for the expression of cytokine genes, that is their functional potential. Here, we show that for the maximal reexpression of cytokine genes, the Ca2+ concentration of the medium has to be greater than 1.5 mM. For RPMI (Roswell Park Memorial Institute 1640), the most commonly used medium, the Ca2+ concentration has to be increased from the regular 0.42 to 1.5 mM.
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