期刊
BIO-PROTOCOL
卷 7, 期 13, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2371
关键词
Glycogen; Trehalose; Yeast; Storage carbohydrate; Cell cycle
类别
资金
- NIH [RO1 GM 119175]
Organisms store carbohydrates in several forms. In yeast, carbohydrates are stored in glycogen (a multi-branched polysaccharide) and in trehalose (a disaccharide). As in other organisms, the amount of stored carbohydrate varies dramatically with physiological state, and accordingly, an assay of stored carbohydrate can help reveal physiological state. Here, we describe relatively easy and streamlined assays for glycogen and trehalose in yeast that can be applied either to a few samples, or in a moderately high-throughput fashion (dozens to hundreds of samples).
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