期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 26, 页码 21631-21638出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b03672
关键词
Staphylococcus aureus; MRSA; Pseudomonas aeruginosa; biofilms; chemotherapy; silver; nanoparticles
资金
- Hospital Research Foundation, Woodville, Australia
- Department of Surgery, Otolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, Australia
- Australian Government Research Training Program Scholarship
- National Health and Medical Research Council, Australia [NHMRC: GNT1090898]
- Florey Medical Research Foundation, Adelaide, Australia
- Freemasons Foundation, Adelaide, Australia
Biofilms are aggregates of bacteria residing in a self-assembled matrix, which protects these sessile cells against external stress, including antibiotic therapies. In light of emerging multidrug-resistant bacteria, alternative strategies to antibiotics are emerging. The present study evaluated the activity of colloidal silver nanoparticles (AgNPs) of different shapes against biofilms formed by Staphylococcus aureus (SA), methicillin-resistant SA (MRSA), and Pseudomonas aeruginosa (PA). Colloidal quasi-spherical, cubic, and star-shaped AgNPs were synthesized, and their cytotoxicity on macrophages (THP-1) and bronchial epithelial cells (Nuli-1) was analyzed by the lactate dehydrogenase assay. The antibiofilm activity was assessed in vitro by the resazurin assay and in an in vivo infection model in Caenorhabditis elegans. Cubic and star-shaped AgNPs induced cytotoxicity, while quasi-spherical AgNPs were not toxic. Quasi-spherical AgNPs showed substantial antibiofilm activity in vitro with 96% (+/- 2%), 97% (+/- 1%), and 98% (+/- 1%) biofilm killing of SA, MRSA, and PA, respectively, while significantly reducing mortality of infected nematodes. The in vivo antibiofilm activity was linked to the accumulation of AgNPs in the intestinal tract of C. elegans as observed by 3D X-ray tomography. Quasi-spherical AgNPs were physically stable in suspension for over 6 months with no observed loss in antibiofilm activity. While toxicity and stability limited the utilization of cubic and star shaped AgNPs, quasi-spherical AgNPs could be rapidly synthesized, were stable and nontoxic, and showed substantial in vitro and in vivo activity against clinically relevant biofilms. Quasi-spherical AgNPs hold potential as pharmacotherapy, for example, as topical treatment for biofiltn-related infections.
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