期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 114, 期 8, 页码 1791-1802出版社
WILEY
DOI: 10.1002/bit.26270
关键词
microfluidic cell sorting; CHO cells; recombinant protein expression; protein secretion; magnetic microparticles
资金
- Kommission fur Technologie und Innovation [12114.1]
- Selexis SA
- Spinomix SA
- University of Lausanne
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure. Biotechnol. Bioeng. 2017;114: 1791-1802. (c) 2017 Wiley Periodicals, Inc.
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