A facile way to prepare functionalized dextran nanogels for conjugation of hemoglobin

Nanogels with several special advantages have been widely applied in protein delivery. However, bio-compatible and biodegradable nanogels used for hemoglobin (Hb) delivery are far less explored. Herein, we developed a facile method to prepare functionalized dextran nanogels for conjugation of Hb. In situ cross-linked and aldehyde group functionalized nanogels (FNGs) were prepared from dextran-g-succinic anhydride-g-dopamine conjugate (Dex-SA-DA) assembly by simple pH adjustion and oxidization in water. Hb was further conjugated into the swelling FNGs by Schiff base reaction under mild condition. The obtained hemoglobin-loaded nanogels (HbNGs) exhibited high stability, oxygen affinity and good hemo-compatibility, suggesting the potential for oxygen carriers. We expected that the designed functionalized nanogels with high stability and loading capacity could bring a new opportunity for protein delivery. (C) 2017 Elsevier B.V. All rights reserved.