期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 45, 期 6, 页码 1696-1705出版社
WILEY
DOI: 10.1002/eji.201445239
关键词
Axl receptor tyrosine kinase; hepatitis B virus (HBV); immune regulation; TAM receptor tyrosine kinase; type I interferons
类别
资金
- Ministry of Science and Technology, Taiwan [NSC97similar to99-2321-B-002-026, NSC97similar to99-2321-B-002-004, NSC97similar to99-2321-B-002-003]
- Institute of Biomedical Sciences, Academia Sinica, Taiwan [CRC101-P03]
Hepatitis B virus (HBV) is known to cause age-dependent infection outcomes wherein most infections during young age result in chronicity. The mechanism underlying the differential outcome remains elusive. By using hydrodynamic injection of the replication-competent pAAV-HBV, we established a mouse model in which HBV persistence was generated in 4-5 w/o C57BL/6 young mice, but not in adult mice over 10 w/o. HBV-tolerant young mice expressed higher interferon (IFN)-/ levels in hepatocytes and intrahepatic plasmacytoid DCs (pDCs) than adult mice after pAAV-HBV injection. Excessive IFN-/ expression in young mice was associated with induction of the Axl regulatory pathway and expansion of intrahepatic Treg cells. In line with these findings, augmented IFN- expression increased Axl expression in the liver and HBV persistence in adult mice, whereas IFN-/ signaling blockage decreased Axl expression and HBV persistence in young mice. Accordingly, Axl overexpression decreased HBV clearance of adult mice whereas Axl silencing enhanced HBV clearance of young mice. In vitro, IFN- priming of pDCs and Axl-overexpressing macrophages enhanced Treg-cell differentiation. These findings suggest that age-dependent HBV chronicity is attributed to IFN--Axl immune regulation, which is selectively induced in young mice by excessive IFN-/ production at early stage of HBV infection.
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