期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 625, 期 -, 页码 30-38出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2017.05.020
关键词
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资金
- Natural Science Foundation of China [81501625, 31171144, 81401765, 81272177]
- State Key Laboratory Grant of Human Factors Engineering [SYFD150051802]
- Advanced Space Medico-Engineering Research Project of China [FTKY201507]
The myogenesis of skeletal muscle has several stages, including satellite cell proliferation, differentiation, fusion and specific muscle formation. Recent studies have shown that myomaker, a muscle-specific transmembrane protein, was critical for myoblasts fusion. However, the regulatory mechanism of myomaker and its effects on myogenesis remain elusive. In this study, miR-491 was identified as a post transcriptional regulator of myomaker, which binds specifically to its 3' untranslated region leading to its down-regulation. At the end of myotube differentiation, the expression levels of miR-491 increased drastically, while myomaker was significantly down-regulated, which indicated that miR-491 shut down the expression of myomaker. Functional studies showed that miR-491 overexpression suppressed muscle cell differentiation and adult muscle regeneration, while the inhibition of miR-491 promoted myotube differentiation. Taken together, our findings identified miR-491 as a novel negative regulator of myogenic differentiation through targeting myomaker. (C) 2017 Published by Elsevier Inc.
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