期刊
BIOSENSORS & BIOELECTRONICS
卷 93, 期 -, 页码 260-266出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.08.099
关键词
Norovirus detection; Sensor design; Sensitivity enhancement; V-trench biosensor; Surface plasmon resonance; Quantum dot
类别
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [26246008]
- Grants-in-Aid for Scientific Research [16K09956, 26246008] Funding Source: KAKEN
A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of auto fluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390 nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01 ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated. (C) 2016 The Authors. Published by Elsevier B.V.
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