期刊
CHEMPHYSCHEM
卷 18, 期 13, 页码 1811-1821出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201700301
关键词
alginate hydrogel; DNA release; enzymes; logic gates; signal-controlled release
资金
- National Science Foundation [CBET-1403208]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1403208] Funding Source: National Science Foundation
Signal-controlled release of DNA from Fe3+-cross-linked alginate hydrogel electrochemically deposited on an electrode surface was studied. The multiple input signals were logically processed with the help of the enzyme-biocatalyzed reactions. Boolean logic gates, OR, AND, INH, were realized with the biocatalytic reactions performed by the enzymes entrapped in the alginate film. Hydrogen peroxide produced by the enzymatic reactions resulted in the degradation of the alginate hydrogel and DNA release. The alginate degradation was facilitated by the formation of free radicals in the Fenton-type reaction catalyzed by iron cations cross-linking the alginate hydrogel. The studied approach is versatile and can be adapted to various chemical signals processed by various enzymes with differently implemented Boolean logic. This work illustrates a novel concept of functional integration of biomolecular computing and actuation.
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