期刊
FOOD & FUNCTION
卷 8, 期 7, 页码 2601-2610出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7fo00236j
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资金
- National Natural Science Foundation of China [31460422, 21167013]
- Natural Science Foundation of Jiangxi Province [20171BBB204029, 20143ACB20006]
- Objective-Oriented Project of State Key Laboratory of Food Science and Technology [SKLF-ZZA-201612]
- Postgraduate Innovation Fund of Nanchang University [CX2016213]
Dihydromyricetin (DMY), a natural flavonoid, was found to effectively inhibit tyrosinase activity in a mixedtype manner with an IC50 value of (3.66 +/- 0.14) x 10(-5) mol L-1. DMY combined with the dietary vitamin D-3 at lower concentrations exhibited a synergistic effect on the inhibition of tyrosinase. The formation of a DMY-tyrosinase complex led to fluorescence quenching and conformational changes of tyrosinase, which was driven mainly by hydrophobic interactions and hydrogen bonds. The molecular simulation further found that DMY inserted into the active pocket of tyrosinase interacted with amino acid residues Tyr78, His85, and Ala323, occupying the catalytic center of tyrosinase to hinder entrance of the substrate, leading to the inhibition of tyrosinase. This study may provide a scientific foundation for screening effective tyrosinase inhibitors.
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