期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 489, 期 2, 页码 89-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.05.088
关键词
Biomineralization; Prismatic layer; Chitinase; Chitin
资金
- Japan Society for the Promotion of Science (JSPS) [JP25850078, JP16K20995]
- Tokyo Ohka Foundation for The Promotion of Science and Technology
- Grants-in-Aid for Scientific Research [16H02998, 15H02149] Funding Source: KAKEN
Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. (C) 2017 Published by Elsevier Inc.
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