期刊
BIORESOURCE TECHNOLOGY
卷 239, 期 -, 页码 197-203出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.005
关键词
Bacillus amyloliquefaciens NX-2S; Poly-(gamma-glutamic acid); Inulin; Non-food fermentation
资金
- National High Technology Research and Development Program of China (863) [2015AA020951]
- National Nature Science Foundation of China [21506098]
- Natural Science Foundation of the Jiangsu [BK20150946]
- Natural Science Research Project in Jiangsu Province [15KJB530007]
This study aimed to develop non-food fermentation for the cost-effective production of poly-(gamma-glutamic acid) (gamma-PGA) using a novel strain of Bacillus amyloliquefaciens NX-2S. The new isolate assimilated inulin more efficiently than other carbohydrates from Jerusalem artichoke, without hydrolytic treatment. To investigate the effect of inulin on gamma-PGA production, the transcript levels of gamma-PGA synthetase genes (pgsB, pgsC, pgsA), regulatory genes (comA, degQ, degS), and the glutamic acid biosynthesis gene (glnA) were analyzed; inulin addition upregulated these key genes. Without exogenous glutamate, strain NX2S could produce 6.85 +/- 0.22 g/L of gamma-PGA during fermentation. Exogenous glutamate greatly enhances the gamma-PGA yield (39.4 +/- 0.38 g/L) and productivity (0.43 +/- 0.05 g/L/h) in batch fermentation. Our study revealed a potential method of non-food fermentation to produce high-value products. (C) 2017 Published by Elsevier Ltd.
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