期刊
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
卷 28, 期 -, 页码 27-34出版社
UNIV CATOLICA DE VALPARAISO
DOI: 10.1016/j.ejbt.2017.05.001
关键词
Alginate operon; Alginate synthesis; Alginate-producing organisms; Applications; Biotechnological resources; Brown algae; Environmental bacteria; Exopolysaccharide matrix; Gels; Linear polysaccharides; Survival strategies
资金
- Direccion de Investigacion, Pontificia Universidad Catolica de Valparaiso Project [DI 037.431/2015]
- INACH Project doctorado [DT_02-13]
- beca de mantencion de postgrado, Vice-rectoria de Investigacion y Estudios Avanzados, Pontificia Universidad Catolica de Valparaiso
Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4 degrees C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production. (C) 2017 Pontificia Universidad Catilica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. This is an open access article under the CC BY-NC-ND license.
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