期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 53, 期 4, 页码 470-475出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0003683817040111
关键词
magnetite nanoparticles; immunochromatographic assay; sensitivity enhancement; test strips; zearalenone; T-2 toxin
资金
- Russian Foundation for Basic Research [15-08-07913_a]
Highly sensitive immunochromatographic test systems were developed for the detection of zearalenone (ZEA) and T-2 toxin (T2T) using magnetite nanoparticles (MNPs) for the labeling. In order to detect an analyte with high sensitivity, the competitive reaction was performed with free specific antibodies, while immune complexes were detected by the reaction with label-conjugated anti-species antibodies. The conditions for the synthesis of magnetite nanoparticles conjugated to anti-species antibodies were optimized. The concentrations of specific reagents that provided the highly sensitive detection of T-2 toxin and zearalenone were found. The instrumental detection limit for the determination of T-2 toxin and zearalenone in baby food samples (oat flakes) was 0.1 and 0.05 ng/mL (2.0 and 1.0 ng/g), respectively. The assay time was 15 min. The results of the present study confirm the possibility of the practical use of magnetite nanoparticles for immunochromatographic assay labeling.
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