期刊
ALLERGOLOGIA ET IMMUNOPATHOLOGIA
卷 45, 期 4, 页码 350-355出版社
ELSEVIER DOYMA SL
DOI: 10.1016/j.aller.2016.10.007
关键词
Allergic rhinitis; Hydrogen-rich saline; Immunofluorescence; IgE; Ovalbumin
资金
- Natural Science Foundation of Tongji Hospital [JYB1302]
Background: Medical gas hydrogen (H-2) has a special role in airway inflammation; however, the effect of H-2 on allergic rhinitis (AR) remains unclear. This study explored the possible roles of H-2 on the pathogenesis of AR and observed the influences of H-2 on cytokines IL-4 and IL-13. Methods: An AR guinea pig model was established by nasal ovatbumin sensitisation. Eighteen guinea pigs were divided into three groups, namely, saline control, AR-sensitised, and hydrogen rich saline (HRS)-treated groups, with each group having six guinea pigs. The frequencies of sneezing and scratching were recorded. The IgE level and cytokine (IL-4 and IL-13) levels in the serum were measured. The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also determined by real-time reverse transcriptase-polymerase chain reaction and Western blot. We also observed the infiltration of cytokine (IL-4 and IL-13) in nasal mucosa by immunofluorescence. Results: The frequencies of sneezing and scratching, as well as the levels of IgE, IL-4, and IL-13, in the serum were higher in the AR group than in the control group (p < 0.01), whereas all these parameters were decreased significantly after HRS treatment (p < 0.05). The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also lower in guinea pigs treated with HRS than those in the AR group (p < 0.05). Conclusions: HRS could affect anti-inflammation in AR and decreased the expression of IL-4 and IL-13. (C) 2016 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.
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