期刊
ELECTROCHIMICA ACTA
卷 244, 期 -, 页码 96-103出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2017.05.089
关键词
Aptamer; Aflatoxin B1; DPV; Alcoholic beverages; Graphene; Methylene blue
资金
- EUPHRATES Program for ERASMUS Mundus Doctoral Fellowship
In this work, we developed an electrochemical aptasensor by using methylene blue (MB) redox probe labeled aptamer as a signaling fragment and functional graphene oxide (FGO) as the signal-enlarging platform. The role of functionalized graphene oxide was not mere to serve as a covalent immobilization support for aptamer sequences, but its catalytic signal amplification behavior towards methylene blue was demonstrated for the first time in the present work. The functionalized graphene oxide was cast on screen-printed carbon electrodes (SPCE), and then the MB-tagged aptamer was covalently immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. Aflatoxin B1 (AFB1) analyte molecule detection was accomplished by the aptamer conjugated redox probe, which undergoes/involves a conformational change in the complex structure of aptamer consequent to AFB1 binding. The proposed assay permitted to detect AFB1 in the linear range of 0.05-6.0 ng mL(-1) with a very low limit of detection (LOD) (0.05 ng mL(-1)). The present principle aptasensor was tested to screen the alcoholic beverage samples for AFB1 detection and good recovery values were obtained. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据