期刊
BIORESOURCE TECHNOLOGY
卷 238, 期 -, 页码 129-138出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.04.041
关键词
Microalgae oil; Human milk fat substitutes (HMFs); n-3 polyunsaturated fatty acids (n-3 PUFAs); Lipase; Acidolysis
资金
- Department of Science and Technology of Fujian Province of China [2015N007]
- Department of Science and Technology of Fuzhou City of Fujian Province of China [2016-G-73]
- 13th Five-year National Innovation and Development of Marine Economy Demonstration Project
Human milk fat substitutes (HMFs) with four kinds of n-3 fatty acid for infant formula were firstly synthesized using triacylglycerols (TAGs) from Nannochloropsis oculata rich in PA at the sn-2 position and free fatty acids (FFAs) from Isochrysis galbana rich in n-3 polyunsaturated fatty acids (n-3 PUFAs-ALA/SDA/ DHA) via solvent-free acidolysis with Novozym 435, Lipozyme 435, TL-IM and RM-IM as biocatalysts. The results show that the resulting HMFs contain total n-3 PUFA of 13.92-17.12% and PA of 59.38-68.13% at the sn-2 position under the optimal conditions ( mole ratio FFAs/TAG 3: 1, 60 degrees C (Novozym 435 and Lipozyme TL-IM) and 50 degrees C (Lipozyme 435 and RM-IM), lipase loading 10%, reaction time 24 h). Moreover, among the tested enzymes, Lipozyme 435, TL-IM, and RM-IM display the fatty acid selectivity towards SDA, LA and ALA, and OA, respectively. Overall, the examined lipases are promising biocatalysts for producing high-value microalgal HMFs in a cost-effective manner. (C) 2017 Elsevier Ltd. All rights reserved.
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