期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 46, 期 -, 页码 134-140出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2017.03.014
关键词
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资金
- Novo Nordisk Foundation
- Joint BioEnergy Institute - US Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- NNF Center for Biosustainability [Synthetic Biology Tools for Yeast] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modifications and transcriptional regulations. The effectiveness of these tools allows researchers to implement genome-wide perturbations, test model-guided genome editing strategies, and perform transcriptional reprogramming perturbations in a more advanced manner than previously possible. In this mini-review we highlight recent studies adopting CRISPR/Cas9 for systems-level perturbations and model-guided metabolic engineering.
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