期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 27, 期 15, 页码 3284-3288出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2017.06.026
关键词
Cationic liposomes; Spacer; Gemini amphiphiles; Transfection; Gene therapy
资金
- President's Program in Support of Leading Scientific Schools [SS-7946.2016.11]
- Russian Foundation for Basic Research [13-04-40183 comfi, 13-04-40181 comfi]
Three novel polycationic gemini amphiphiles with different spacers were developed and evaluated in terms of their physiochemical properties and transfection efficiencies. Cationic liposomes formed by these amphiphiles and the helper lipid DOPE were able to successfully condense DNA, as shown by gel mobility shift and ethidium bromide intercalation assays. Transfection activity of the liposomes was superior to Lipofectamine((R)) 2000 and was dependent on spacer structure, hydrophobicity, and nucleic acid type (pDNA or siRNA). We demonstrated that the cationic liposomes 2X6/DOPE and 2X7/DOPE are potential non-toxic vehicles for gene delivery. (C) 2017 Elsevier Ltd. All rights reserved.
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