期刊
BIOTECHNOLOGY LETTERS
卷 39, 期 8, 页码 1237-1244出版社
SPRINGER
DOI: 10.1007/s10529-017-2357-7
关键词
D-Aminoacylase; Bacillus subtilis; Expression vector; Gene deletion; Luciferase; P-malA promoter
资金
- National Nature Science Foundation of China [31370089, 21506244]
- State Key Development 973 Program for Basic Research of China [2013CB733600]
- Nature Science Foundation of Tianjin City (CN) [16JCYBJC23500]
- Key Projects in the Tianjin Science & Technology Pillar Program [11ZCZDSY 08600]
Objectives To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. Results An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the PmalA-derived system, mutagenesis was employed by gradually shortening the length of PmalA promoter and altering the spacing between the predicted MalR binding site and the-35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the PmalA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the PhpaII system. Conclusions A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.
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