期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 27, 期 8, 页码 1500-1512出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1705.05021
关键词
Bacillus sp PZ-1; siderophore; response surface methodology; Pb; phytoextraction
资金
- Natural Science Foundation of Heilongjiang Province, China [D2016001]
In this study, the siderophore-producing characteristics and conditions of Bacillus sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in Brassica juncea were determined. Results of single factor experiment showed that glucose, pH, and Pb(NO3)(2) could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at the glucose concentration of 21.84 g/l, pH 6.18, and Pb(NO3) 2 concentration of 245.04 mu mol/l. The type of siderophore was hydroxamate and its concentration in the fermentation broth amounted to 32.24 mu g/ml. Results of pot experiments indicated that the siderophores enhanced B. juncea to assimilate more Pb from soil with the uptake ratio from 1.04 to 2.74, and to translocate more Pb from underground to overground with the TF values from 1.21 to 1.48. The results revealed that Bacillus sp. PZ-1 could produce abundant siderophores and might be potentially used to augment the phytoextraction of Pb from soil.
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