期刊
BIOMEDICAL OPTICS EXPRESS
卷 8, 期 9, 页码 4160-4171出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.004160
关键词
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资金
- Research Grants Council of the Hong Kong Special Administrative Region of China [HKU 17259316, HKU 17207715, HKU 17208414, HKU 720112E, C7047-16G]
- University Development Funds of HKU
Apart from the spatial resolution enhancement, scaling of temporal resolution, equivalently the imaging throughput, of fluorescence microscopy is of equal importance in advancing cell biology and clinical diagnostics. Yet, this attribute has mostly been overlooked because of the inherent speed limitation of existing imaging strategies. To address the challenge, we employ an all-optical laser-scanning mechanism, enabled by an array of reconfigurable spatiotemporally-encoded virtual sources, to demonstrate ultrafast fluorescence microscopy at line-scan rate as high as 8 MHz. We show that this technique enables high-throughput single-cell microfluidic fluorescence imaging at 75,000 cells/second and high-speed cellular 2D dynamical imaging at 3,000 frames per second, outperforming the state-of-the-art high-speed cameras and the gold-standard laser scanning strategies. Together with its wide compatibility to the existing imaging modalities, this technology could empower new forms of high-throughput and high-speed biological fluorescence microscopy that was once challenged. (C) 2017 Optical Society of America
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