期刊
ARABIAN JOURNAL OF CHEMISTRY
卷 12, 期 7, 页码 1108-1120出版社
ELSEVIER
DOI: 10.1016/j.arabjc.2017.12.002
关键词
Green synthesis; Silver nanoparticles; Ganoderma applanatum; Antimicrobial; Antioxidant
This study presents the use of basidiomycete extracts as an effective platform for green synthesis of silver nanoparticles (AgNPs). Out of seven basidiomycete species, Ganoderma applanatum displayed the highest antimicrobial properties against the tested pathogens. Thus, G. applanatum methanol crude extract was fractionated using column chromatography, and the obtained fractions were subjected to an antimicrobial assay followed by phytochemical analyses using high-performance liquid chromatography to select the best fraction for synthesis of AgNPs. Fraction 3 displayed potent antimicrobial activities as evidenced by its high phenolic content, and thus was used for AgNP biosynthesis. The G. applanatum fraction 3-synthesized AgNPs were then characterized using various microscopy, spectroscopy and X-ray diffraction techniques. The characteristic features of the synthesized AgNPs indicated the spherical shape of AgNPs with an average size of 20-25 nm. The synthesized AgNPs exhibit high antioxidant capacity, in vitro antibacterial activity against Staphylococcus aureus and Escherichia coli, and in vivo antifungal properties against Botrytis cinerea and Colletotrichum gloeosporioides in tomato and strawberry leaflet assays, respectively. Our results demonstrated that G. applanatum can be efficiently used in synthesis of AgNPs with potent antimicrobial properties, which can be used for both clinical and agrochemical purposes. (C) 2017 Production and hosting by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据