期刊
ACS NANO
卷 11, 期 8, 页码 8084-8091出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b02893
关键词
multicolor super-resolution imaging; super-resolution optical fluctuation imaging; blue and carmine small polymer dots; photoblinking; bioimaging
类别
资金
- National Natural Science Foundation of China [61475010]
- Key Program of National Natural Science Foundation of China [61335001, 21573013, 21390412, 31271423, 31327901]
- Thousand Young Talents Program
- 863 Program [SS2015AA020406]
Advances in the development of small photoblinking semiconducting polymer dots (Pdots) have attracted great interest for use in super-resolution microscopy. However, multicolor super-resolution imaging using conventional small photoblinking Pdots remains a challenge due to their limited color choice, broad emission spectrum, and heavy spectrum crosstalk. Here, we introduce two types of small photoblinking Pdots with different colors and relatively narrow emission spectra: blue PFO Pdots and carmine PFTBTS Pdots for blinking-based statistical nanoscopy. Both of these probes feature ultrahigh single-particle brightness, very strong photostability, superior biocompatibility, and robust fluorescence fluctuation. In addition, these small photoblinking Pdots serve as excellent labels for dual-color super resolution optical fluctuation imaging (SOFT) of specific subcellular structures, indicating their promise for long-term multicolor SOFI nanoscopy with high spatiotemporal resolution.
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