期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 46, 期 -, 页码 106-113出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2017.02.009
关键词
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资金
- MINECO
- German Research Foundation (DFG) [PA 794/25-1]
- Chinesisch-Deutsches Zentrum fur Wissenschaftsforderung [GZ 905]
Due to its enormous relevance the corona formation of adsorbed proteins around nanoparticles is widely investigated. A comparison of different experimental techniques is given. Direct measurements of proteins, such as typically performed with mass spectrometry, will be compared with indirect analysis, in which instead information about the protein corona is gathered from changes in the properties of the nanoparticles. The type of measurement determines also whether before analysis purification from unbound excess proteins is necessary, which may change the equilibrium, or if measurements can be performed in situ without required purification. Pros and contras of the different methods will be discussed.
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