期刊
BIORESOURCE TECHNOLOGY
卷 238, 期 -, 页码 650-656出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.04.107
关键词
Microalgae; CO2 fixation; Nuclear irradiation; Domestication; Ultrastructure
资金
- National key research and development program-China [2016YFB0601003]
- National Natural Science Foundation-China [51476141]
Spirulina sp. was mutated by gamma-rays from Co-60 nuclear irradiation to improve growth and CO2 fixation rate under 15 vol.% CO2 ( in flue gas from a power plant). Mutants with enhanced growth phenotype were obtained, with the best strain exhibiting 310% increment in biomass yield on day 4. The mutant was then domesticated with elevated CO2 concentration, and the biomass yield increased by 500% after domestication under 15 vol.% CO2, with stable inheritance. Ultrastructure of Spirulina sp. shows that the fractal dimension of Spirulina cells decreased by 23% after mutation. Pore size in the cell wall of Spirulina mutant increased by 33% after 15 vol.% CO2 domestication. This characteristic facilitated the direct penetration of CO2 into cells, thus improving CO2 biofixation rate. (C) 2017 Elsevier Ltd. All rights reserved.
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