期刊
BIOELECTROCHEMISTRY
卷 116, 期 -, 页码 10-16出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2017.03.003
关键词
Microbial fuel cell; Charge transfer; Acetate; Glyoxylate cycle; Carbohydrate synthesis, yeast
资金
- National Science Fund of the Ministry of Education and Science of Bulgaria [DFNI E02/14/2014]
This study provides a new insight into our understanding of yeast response to starvation conditions (sole acetate as carbon source) and applied polarization and offers important information about the role of the glyoxylate cycle in the carbohydrate synthesis and extracellular charge transfer processes in biofuel cells. The biosynthetic capabilities of yeast C melibiosica 2491 and the up/down-regulation of the glyoxylate cycle are evaluated by modifying the cellular metabolism by feedback inhibition or carbohydrate presence and establishing the malate dehydrogenase activity and carbohydrate content together with the electric charge passed through bioelectrochemical system. 10 mM malate leads to a decrease of the Produced quantity of electricity with ca. 55%. At the same time, 24-times lower intracellular malate dehydrogenase activity is established. At polarization conditions the glyoxylate pathway is up-regulated and huge amount of malate is intra-converted into oxaloacetate. The yeasts are able to synthesize carbohydrates from acetate and a part of them is used for the electricity generation. It is recognized that the enhanced charge transfer in acetate fed yeast-based biofuel cell is implemented by secreted endogenous mediator and changes in the cellular surface redox activity depending on the addition of carbohydrate in the medium. (C) 2017 Elsevier B.V. All rights reserved.
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