期刊
JOURNAL OF PHARMACEUTICAL ANALYSIS
卷 7, 期 3, 页码 176-180出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpha.2017.01.003
关键词
Valrubicin-DNA interactions; Spectroscopy; Cyclic voltammetry
资金
- Research Council, Gachsaran Branch, Islamic Azad University under IAUG Individual Research Grant [24094]
- University of Malaya Research Grant [UMRG RP020C-16SUS]
In this study, the molecular interactions between valrubicin, an anticancer drug, and fish sperm DNA have been studied in phosphate buffer solution (pH 7.4) using UV-Vis spectrophotometry and cyclic voltammetry techniques. Valrubicin intercalated into double stranded DNA under a weak displacement reaction with methylene blue (MB) molecule in a competitive reaction. The binding constant (k(b)) of valrubicin-DNA was determined as 1.75x10(3) L/mol by spectrophotometric titration. The value of non-electrostatic binding constant (k(t)(0)) was almost constant at different ionic strengths while the ratio of k(t)(0)/k(b) increased from 4.51% to 23.77%. These results indicate that valrubicin binds to ds-DNA via electrostatic and intercalation modes. Thermodynamic parameters including Delta H-0,Delta S-0 and Delta G(0) for valrubicin-DNA interaction were determined as -25.21x10(3) kJ/mol, 1.55x10(2) kJ/mol K and -22.03 kJ/mol, respectively. Cyclic voltammetry study shows a pair of redox peaks for valrubicin at 0.45 V and 0.36 V (vs. Ag/AgCl). The peak currents decreased and peak positions shifted to positive direction in the presence of DNA, showing intercalation mechanism due to the variation in formal potential.
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