期刊
BIO-PROTOCOL
卷 7, 期 15, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2429
关键词
Mouse; Muller glial cells; Stem cells; Retinal cell isolation; Primary cell culture; Worthington Papain Kit
类别
资金
- National Institute of General Medical Sciences [P20GM103453]
- University of Louisville School of Medicine [E0819]
- Research to Prevent Blindness
Muller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Muller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Muller cells.
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