期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/ncomms15269
关键词
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资金
- Office of Science (BER), US Department of Energy [DE-SC0008397]
- NCI Cancer Center Nanotechnology Excellence Grant (CCNE-TR) [U54 CA119367]
- Calbrain Programme, a Neurotechnology Programme of California [CA151459]
- National Natural Science Foundation of China [81573383, 81373254, 21390402]
- NSFHP [2014CFB704]
- International S&T Cooperation Programme of China [2015DFA30440, 2014DFB30020]
- Ministry of Science and Technology of China [2012ZX10004801-003-011]
- Chinese Ministry of Education [313040]
- Academic Award for Excellent PhD Candidates - Ministry of Education of China [5052012306001]
- Fundamental Research Funds for the Central Universities
- Wuhan University School of Medicine
Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. Here, we report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with similar to 50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. In addition, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body.
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