期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms16087
关键词
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资金
- National Institutes of Health [R01-GM115622, R01-CA207701, R01-AG045183, R01-AT009050, R21-EB022302, DP1-DK113644, R01-GM120033, R01-NS100893]
- Welch Foundation [Q-1912]
- Cancer Prevention and Research Institute of Texas [CPRIT R1104, RP130573]
- Whitehall Foundation Research Grant [2015-05-54]
- Curtis Hankamer Basic Research Fund at Baylor College of Medicine
- Caroline DeLuca Scholarship
- United States Department of Agriculture, Agricultural Research Service [58-6250-0-008]
- IDDRC Microscopy Core (Intellectual and Developmental Disabilities Research Grant from the Eunice Kennedy Shriver National Institute of Child Health and Human Development) [P30HD024064, 1U54 HD083092]
- Optical Imaging and Vital Microscopy core
- Cytometry and Cell Sorting Core at Baylor College of Medicine
- NIH [AI036211, CA125123, RR024574]
Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.
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