期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-017-01962-x
关键词
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资金
- European Research Council under the 7th Framework Program (FP7) [260787]
- European Union's Horizon research and innovation program [676556]
- Labex EpiGenMed, an Investments for the future program [ANR-10-LABX-12-01]
- Spanish Ministry of Economy and Competitiveness [BFU2013-47736-P]
- Centro de Excelencia Severo Ochoa [SEV-2012-0208]
- French National Research Agency [ANR-10-INBS-04]
- European Research Council under the 7th Framework Program (ERC) [609989]
At the kilo-to megabase pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate to form nuclear compartments. Here, we combine high-content super-resolution microscopies with state-of-theart DNA-labeling methods to reveal the variability in the multiscale organization of the Drosophila genome. We find that association frequencies within TADs and between TAD borders are below similar to 10%, independently of TAD size, epigenetic state, or cell type. Critically, despite this large heterogeneity, we are able to visualize nanometer-sized epigenetic domains at the single-cell level. In addition, absolute contact frequencies within and between TADs are to a large extent defined by genomic distance, higher-order chromosome architecture, and epigenetic identity. We propose that TADs and compartments are organized by multiple, small-frequency, yet specific interactions that are regulated by epigenetics and transcriptional state.
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