期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms13972
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资金
- Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC 0001035]
- European Research Council [338895]
- Biotechnology and Biological Sciences Research Council (BBSRC UK) [BB/G021546/1]
- Engineering and Physical Sciences Research Council (EPSRC UK)
- PARC
- EPSRC [EP/I012060/1]
- National Science Foundation [DGE-1143954]
- BBSRC [BB/G021546/1] Funding Source: UKRI
- EPSRC [EP/I012060/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G021546/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I012060/1, 1623768] Funding Source: researchfish
Photosynthesis uses a limited range of the solar spectrum, so enhancing spectral coverage could improve the efficiency of light capture. Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the cytoplasmic side of the RC complex. Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP-RC intermolecular distance and spectral overlap between the emission of YFP and the visible-region (QX) absorption bands of the RC allow energy transfer via a Forster mechanism, with an efficiency of 40 +/- 10%. This proof-of-principle study demonstrates the feasibility of increasing spectral coverage for harvesting light using non-native genetically-encoded light-absorbers, thereby augmenting energy transfer and trapping in photosynthesis.
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