期刊
INTERNATIONAL JOURNAL OF BIOPRINTING
卷 3, 期 2, 页码 138-146出版社
WHIOCE PUBLISHING PTE LTD, SINGAPORE
DOI: 10.18063/IJB.2017.02.008
关键词
retina; bioprinting; age-related macular degeneration; tissue engineering
资金
- NTU Start up Grant
- NTUNHG Innovation Collaboration Grant [ARG-14009]
In this article, a hybrid retina construct was created via three-dimensional (3D) bioprinting technology. The construct was composed of a PCL ultrathin membrane, ARPE-19 cell monolayer and Y79 cell-laden alginate/pluronic bioink. 3D bioprinting technology was applied herein to deliver the ARPE-19 cells and Y79 cell-laden bioink to ensure homogeneous ARPE-119 cell seeding; subsequently, two distinctive Y79 cell-seeding patterns were bioprinted on top of the ARPE-19 cell monolayer. The bioprinted ARPE-19 cells were evaluated by prestobluc assay, F-actin, and hematoxylin/cosin (HE) staining, and then the cells were observed under laser scanning and invert microscopy for 114 days. The Y79 cells in alginatc/pluronic bioink after bioprinting had been closely monitored for 7 days. Live/dead assay and scanning electrical microscopy (SEM) were employed to investigate Y79 cell viability and morphology. Both the ARPE-19 and Y79 cells were in excellent condition, and the successfully bioprinted retina model could be utilized in drug delivery, disease mechanism and treatment method discoveries.
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