期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-017-01724-9
关键词
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资金
- Collaborative Research Centre 992 Medical Epigenetics (DFG) [SFB 992/2 2016]
- German Federal Ministry of Education and Research (BMBF) [031 A538A RBC (de.NBI)]
- Deutsche Forschungsgemeinschaft [SFB 992]
- DFG project [GI 747/2-1, PR 1668/1-1, HE 2073/5-1]
- BIOSS Centre for Biological Signalling Studies
- Innovationsfonds Baden-Wurttemberg
- DZHK [B 15-005]
Storage of chromatin in restricted nuclear space requires dense packing while ensuring DNA accessibility. Thus, different layers of chromatin organization and epigenetic control mechanisms exist. Genome-wide chromatin interaction maps revealed large interaction domains (TADs) and higher order A and B compartments, reflecting active and inactive chromatin, respectively. The mutual dependencies between chromatin organization and patterns of epigenetic marks, including DNA methylation, remain poorly understood. Here, we demonstrate that establishment of A/B compartments precedes and defines DNA methylation signatures during differentiation and maturation of cardiac myocytes. Remarkably, dynamic CpG and non-CpG methylation in cardiac myocytes is confined to A compartments. Furthermore, genetic ablation or reduction of DNA methylation in embryonic stem cells or cardiac myocytes, respectively, does not alter genome-wide chromatin organization. Thus, DNA methylation appears to be established in preformed chromatin compartments and may be dispensable for the formation of higher order chromatin organization.
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