期刊
BIOSENSORS & BIOELECTRONICS
卷 94, 期 -, 页码 536-543出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.03.039
关键词
Two-photon; NIR emission; Ultra-low detection limit; Hydrogen peroxide; Cell imaging
类别
资金
- National Natural Science Foundation of China [21136002, 21421005, 21376039, 21422601, 21406028]
- 973 Program of China [2013CB733702]
- 863 program of China [2013AA020202]
Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the H2O2 is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring H2O2 in vivo. In this work, a two-photon excitation (860 nm) NIR fluorescent turn-on probe TPNR-H2O2 for H2O2 based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40 min), excellent sensitivity (DL 72.48 nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-H2O2 clearly demonstrated that the probe could be applied to imaging intracellular H2O2 for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous H2O2 with promising biological application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据