期刊
EMBO REPORTS
卷 18, 期 8, 页码 1367-1381出版社
WILEY
DOI: 10.15252/embr.201643535
关键词
CLAVATA; CLE45; MAKR5; receptor kinase; SERK
资金
- Swiss National Science Foundation [31003A_166394, 31003A_156920]
- German Research Foundation (DFG) [CRC 1101-D01]
- Human Frontier Science Program Career Development Award
- European Molecular Biology Organization (EMBO) Young Investigator program
- EMBO long-term post-doctoral fellowships
- Swiss National Science Foundation (SNF) [31003A_166394, 31003A_156920] Funding Source: Swiss National Science Foundation (SNF)
Arabidopsis root development is orchestrated by signaling pathways that consist of different CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptide ligands and their cognate CLAVATA (CLV) and BARELY ANY MERISTEM (BAM) receptors. How and where different CLE peptides trigger specific morphological or physiological changes in the root is poorly understood. Here, we report that the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root-active CLE peptides. We uncover BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface. In contrast to other plant peptide receptors, we found no evidence that SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) proteins act as coreceptor kinases in CLE45 perception. CRN stabilizes BAM3 expression and thus is required for BAM3-mediated CLE45 signaling. Moreover, protophloem-specific CRN expression complements resistance of the crn mutant to root-active CLE peptides, suggesting that protophloem is their principal site of action. Our work defines a genetic framework for dissecting CLE peptide signaling and CLV/BAM receptor activation in the root.
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