期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms14843
关键词
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资金
- King Abdullah University of Science and Technology (KAUST)
- Academic Excellence Alliance (AEA)
- Academic Partnership Program (APP)
- Medical Research Council
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council UK Regenerative Medicine Plat form Hub 'A Hub for Engineering and Exploiting the Stem Cell Niche' [MR/K026666/1]
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- Marie Curie actions FP7 through the Intra-European Marie Curie Fellowship [275005]
- Individual Marie Sklodowska-Curie Fellowship 'RADoTE' [701664]
- Whitaker International Program, Institute of International Education, United States of America
- ERC Seventh Framework Programme Consolidator grant Naturale CG'' [616417]
- Individual Marie Sklodowska-Curie Fellowship 'IMAGINE' [701713]
- Wellcome Trust [098411/Z/12/Z] Funding Source: Wellcome Trust
- MRC [MR/K026666/1] Funding Source: UKRI
- Medical Research Council [MR/K026666/1] Funding Source: researchfish
- Marie Curie Actions (MSCA) [701664, 701713] Funding Source: Marie Curie Actions (MSCA)
The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy.
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