期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms14772
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资金
- Dutch Technology Foundation STW
- Foundation for Fundamental Research on Matter (FOM)
- Netherlands Organisation for Scientific Research (NWO) [NWO-NANO 11421]
- NWO [NWO-ALW-VICI 865.10.010, NWO-ALWVIDI 864.12.008]
- European Research Council (ERC) [336291]
- European Molecular Biology Organization (EMBO) Long-Term Fellowship (EMBO) [ALTF 884-2011]
- Marie Curie IEF (FP7-PEOPLE-IEF)
- Deutsche Forschungsgemeinschaft Emmy-Noether Programm [Ml1923/1-1]
- European Research Council (ERC) [336291] Funding Source: European Research Council (ERC)
The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.
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