期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 28, 页码 24118-24125出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b05981
关键词
pancreatic carcinoma; biomarker; multiple miRNA detection; electrochemical biosensor; DNA tetrahedral nanostructures
资金
- National Key Research and Development Program of China [2016YFC0100600]
- Ministry of Science and Technology of China [2015BAI02B02]
- Chinese National Natural Science Foundation [21605152, 21605153]
- Science and Technology Service Network Initiative, CAS [KFJ-EW-STS-140, KFJ-EW-STS-096]
- Shanghai Municipal Science and Technology Commission [15441905000, 16DZ1930700]
- Youth Innovation Promotion Association CAS [2017356]
- Shanghai Sailing Program [15YF1413300]
Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability to detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes to enhance the detection sensitivity as well as a disposable 16-channel screen-printed gold electrode (SPGE) detection platform to enhance the detection efficiency, we were able to simultaneously detect four PC -related miRNAs: miRNA21, miRNA155, miRNA196a, and miRNA210. The detection sensitivity reached to as low as 10 fM. We then profiled the serum levels of the four miRNAs for PC patients and healthy individuals with our multiplexing electrochemical biosensor. Through the combined analyses of the four miRNAs, our results showed that PC patients could be discriminated from healthy controls with fairly high sensitivity. This multiplexing PCR-free miRNA detection sensor shows promising applications in early diagnosis of PC disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据