Establishment of a rat model of chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) induced by immunization with a novel peptide T2

Background: The exact etiological mechanism of Chronic Prostatitis/chronic pelvic pain syndrome (CP/ CPPS) is still unclear however autoimmunity is the most valid theory. We developed a rat model of Chronic Prostatitis/chronic pelvic pain syndrome by using a novel peptide (T2) isolated from TRPM8. This model might be beneficial in elucidating mechanisms involved in the pathogenesis of Chronic Prostatitis/ Chronic Pelvic Pain Syndrome (CP/CPPS). Methods: 40 male Sprague-Dawley rats with an average weight of 180-220 g were equally distributed into five groups. The normal control group was injected with normal saline (.9% NACL), the CFA group with CFA, AL(OH)3 group was given AL(OH)3 injection, T2 group using a novel peptide T2 and T2 + AL(OH) 3 + CFA group was injected with T2 + AL(OH)3 + CFA. Dosing to all rat groups were injected subcutaneously. Hematoxylin and eosin staining and Immunohistochemistry were used to investigate inflammatory cell infiltration and IL-1 beta in the prostate tissue respectively. ELISA technique was used to measure the serum level of CRP and TNF-alpha. T-test was used to analyze the results. Results: Maximum infiltration of inflammatory cells and the highest level of IL-1 beta in the prostate tissue was observed in T2 + AL(OH)3 + CFA group as revealed by histopathology and Immunohistochemistry, respectively. Furthermore, T2 + AL(OH) 3 + CFA group attained the peak value of serum TNF-alpha and CRP as determined by ELISA technique. Conclusion: Our results demonstrated that T2 in combination with AL(OH) 3 and CFA induced severe Prostatitis in rats. We believe that our present model will be highly beneficial for investigation of the pathophysiology of Chronic Prostatitis/Chronic Pelvic Pain Syndrome. (C) 2017 Elsevier Masson SAS. All rights reserved.