Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1

YAP and its neuronal isoform YAPdeltaC are implicated in various cellular functions. We found that expression of YAPdeltaC during development, but not adulthood, rescued neurodegeneration phenotypes of mutant ataxin-1 knock-in (Atxn1-KI) mice. YAP/YAPdeltaC interacted with RORa via the second WW domain and served as co-activators of its transcriptional activity. YAP/YAPdeltaC formed a transcriptional complex with RORa on cis-elements of target genes and regulated their expression. Both normal and mutant Atxn1 interacted with YAP/YAPdeltaC, but only mutant Atxn1 depleted YAP/YAPdeltaC from the RORa complex to suppress transcription on short timescales. Over longer periods, mutant Atxn1 also decreased RORa in vivo. Genetic supplementation of YAPdeltaC restored the RORa and YAP/YAPdeltaC levels, recovered YAP/YAPdeltaC in the RORa complex and normalized target gene transcription in Atxn1-KI mice in vivo. Collectively, our data suggest that functional impairment of YAP/ YAPdeltaC by mutant Atxn1 during development determines the adult pathology of SCA1by suppressing ROR alpha-mediated transcription.