期刊
NATURE COMMUNICATIONS
卷 8, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/ncomms14690
关键词
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资金
- Biotechnology and Biological Sciences Research Council (U.K.) New Investigator grant [BB/M024458/1]
- ScienceCampus Halle-Plant-based Bioeconomy
- Landesgraduiertenforderung Sachsen-Anhalt
- Engineering and Physical Sciences Research Council (U.K.) [EP/G03706X/1]
- Royal Society Dorothy Hodgkin Fellowship
- William R. Miller Junior Research Fellowship (St Edmund Hall, Oxford)
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) [DI 1794/3-1]
- Leibniz Association
- state of Saxony Anhalt
- Deutsche Forschungsgemeinschaft (DFG) Graduate Training Center [GRK1026]
- Leibniz Institute of Plant Biochemistry (IPB) at Halle, Germany
- European Cooperation in Science and Technology (COST) Action [BM1307]
- Leibniz Institute of Plant Biochemistry (IPB)
- BBSRC [BB/M024458/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M024458/1] Funding Source: researchfish
Crop yield loss due to flooding is a threat to food security. Submergence-induced hypoxia in plants results in stabilization of group VII ETHYLENE RESPONSE FACTORs (ERF-VIIs), which aid survival under these adverse conditions. ERF-VII stability is controlled by the N-end rule pathway, which proposes that ERF-VII N-terminal cysteine oxidation in normoxia enables arginylation followed by proteasomal degradation. The PLANT CYSTEINE OXIDASEs (PCOs) have been identified as catalysts of this oxidation. ERF-VII stabilization in hypoxia presumably arises from reduced PCO activity. We directly demonstrate that PCO dioxygenase activity produces Cys-sulfinic acid at the N terminus of an ERF-VII peptide, which then undergoes efficient arginylation by an arginyl transferase (ATE1). This provides molecular evidence of N-terminal Cys-sulfinic acid formation and arginylation by N-end rule pathway components, and a substrate of ATE1 in plants. The PCOs and ATE1 may be viable intervention targets to stabilize N-end rule substrates, including ERF-VIIs, to enhance submergence tolerance in agriculture.
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