期刊
ACS NANO
卷 11, 期 7, 页码 6566-6573出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b07097
关键词
DNA nanotechnology; DNA origami; conformational dynamics; molecular crowding; depletion forces; single-molecule biophysics
类别
资金
- NSF [1351159, 1228104, 1516976]
- NIH [R01-GM083055, R21-CA174583]
- Center for Emergent Materials (NSF) [DMR-1420451]
- Ohio State University Institute for Materials Research
- Center for Exploration of Novel Complex Materials (ENCOMM)
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1516976] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1351159] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1228104] Funding Source: National Science Foundation
The ability to self-assemble nanodevices with programmed structural dynamics that can sense and respond to the local environment could enable transformative applications in fields including molecular robotics, nanomanufacturing, and nanomedicine. The responsive function of biomolecules is often driven by alterations in conformational distributions mediated by highly sensitive interactions with the local environment. Here, we mimic this approach by engineering inherent nanoscale structural dynamics (nanodynamics) into a DNA device that exhibits a distribution of conformations including two stable states separated by a transition state where the energy barrier height is on the scale of the thermal energy, k(B)T = 4.1 pN.nm, enabling spontaneous transitions between states. We further establish design principles to regulate the equilibrium and kinetic behavior by substituting a few DNA strand components. We use single-molecule Forster resonance energy transfer measurements to show these nanodynamic properties are sensitive to sub-piconewton depletion forces in the presence of molecular crowding agents, and the device can measure depletion forces with a resolution of similar to 100 fN. We anticipate that this approach of engineering nanodynamic DNA devices will enable molecular scale systems that sense and respond to their local environment with extremely high sensitivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据