期刊
ONCOLOGY LETTERS
卷 14, 期 5, 页码 5581-5584出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2017.6860
关键词
curcumin; MCF-7; proliferation; apoptosis; nuclear factor-kappa-light-chain-enhancer of activated B cells
类别
资金
- Central Research Laboratory, The Second Hospital of Shandong University (Shandong, China)
- National Natural Science Foundation of China [81400072]
- Natural Science Foundation of Shandong Province [ZR2016HM67, 2013HQ047, ZR2013HM103]
- Science and Technology Development Project of Shandong [2016GSF201044, 2016GSF201203]
- Medical and Health Science Technology Development Plan Project of Shandong Province [2015WS0307]
The present study investigated the inhibitory effect of curcumin on human breast cancer MCF-7 cells and investigated the potential underlying molecular mechanisms. MCF-7 cells were cultured with curcumin at different concentrations and time points. The effects of curcumin treatment on breast cancer cell proliferation were studied using a MTT assay. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to assess the mRNA and protein expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax), nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-kappa B) and inhibitor of NF-kappa B-alpha (I kappa Ba). The proliferation of MCF-7 cells in the group treated with curcumin was markedly decreased compared with the control, with the greatest inhibitory effect at a concentration of 20 mu M. The expression of Bax mRNA was increased and Bcl-2 mRNA expression was decreased compared with the control. Additionally, protein expression of NF-kappa B and I kappa B was increased. The data indicate that curcumin is able to inhibit breast cancer cell proliferation, possibly by regulating the NF-kappa B signaling pathway.
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