期刊
CURRENT MICROBIOLOGY
卷 74, 期 9, 页码 1089-1099出版社
SPRINGER
DOI: 10.1007/s00284-017-1289-8
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类别
资金
- European project BIOCOMES [FP7-612713]
- Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya [2014-FI-B00367]
This work focuses on the biological understanding of the biocontrol agent Bacillus amyloliquefaciens CPA-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. The tolerance of CPA-8 to grow under different pH-temperature and water activity (a (w))-temperature conditions was widely demonstrated. Regarding the pH results, optimum growth at the evaluated conditions was observed at 37 A degrees C and pH between 7 and 5. On the contrary, the slowest growth was recorded at 20 A degrees C and pH 4.5. Moreover, the type of solute used to reduce a (w) had a great influence on the minimum a (w) at which the bacterium was able to grow. The lowest a (w) values for CPA-8 growth in media modified with glycerol and glucose were 0.950 and 0.960, respectively. Besides, the lowest a (w) for CPA-8 growth increased when the temperature decreased to 20 A degrees C, at which CPA-8 was not able to grow at less than 0.990 a (w), regardless of the type of solute. Antibiotic susceptibility tests were carried out to determine which antibiotic could affect the behavior of the bacteria and revealed that CPA-8 was clearly resistant to hygromycin. Finally, a PCR amplification assay to detect the presence of enterotoxic genes from Bacillus cereus in CPA-8 was also performed. CPA-8 gave negative results for all the genes tested except for nheA gene, which is not enough for the toxicity expression, suggesting that fruit treated with this antagonist will not be a potential vehicle for foodborne illnesses.
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